LIPID PEROXIDATION AND EXPRESSION OF PARAOXONASE-1 (PON-1) GENE IN PATIENTS WITH PREECLAMPSIA
DOI:
https://doi.org/10.47941/ijhs.830Keywords:
Preeclampsia, Lipid Peroxidation, Paraoxonase-1 Oxidative Stress, MalondialdehydeAbstract
Purpose: To evaluate lipid peroxidation and expression of paraoxonase-1 (pon-1) gene in patients with preeclampsia.
Methodology: The study comprised of 160 participants, in which 80 were normotensive pregnant women (Group A) and 80 were preeclamptic pregnant women (Group B). Serum MDA levels and PON-1 gene activity were estimated. The expression of data was subjected to statistical analysis using SPSS version 20.0. One-way analysis of variance (ANOVA) was used to compare the test samples, p value <0.05 was considered significant.
Findings: MDA levels were significantly elevated in preeclamptic pregnant women when compared to normotensive. Also, PON-1 gene expression levels were significantly decreased in preeclamptic pregnant women when compared to normotensive.
Unique contribution to theory, practice and policy: The significant increase in the plasma level of Malondialdehyde and decreases expression of PON-1 gene could be responsible for the presence of oxidative stress and its associated complications in PE patients.
Downloads
References
Aldridge, W. N. (1953). Serum esterases. 1. Two types of esterase (A and B) hydrolysing p-nitrophenyl acetate, propionate and butyrate, and a method for their determination. Biochemical Journal. Vol. 53, Issue 1, pp 110–117.
Al-Kuraishy, H. M., Al-Gareeb, A. I. & Al-Maiahy, T.J. (2018) Concept and connotation of oxidative stress in preeclampsia. J Lab Physicians. Vol. 10, pp 276-82.
Babacan, F., Isik, B. & Bingol, B. (2011) Changes in serum paraoxonase activity, calcium and lipid profiles in preeclampsia, a priliminary study. Journal of Turkish Society of Obstetrics and Gynecology. Vol. 8. Issue 3, pp 169–174.
Bellamy, L., Casas, J. P., Hingorani, A. D., & Williams, D. J. (2007): “Pre-eclampsia and risk of cardiovascular disease and cancer in later life: systematic review and meta-analysis,” British Medical Journal. Vol. 335. Issue 7627, pp 974–977.
Browne, R. W., Koury, S. T., Marion, S., Wilding, G., Muti, P. & Trevisan, M. (2006) Accuracy and Biological Variation of Human Serum Paraoxonase 1 Activity and Polymorphism (Q192R) by Kinetic Enzyme Assay. Clinical Chemistry. Vol. 53. Issue 2, pp 310–317.
Costa, L. G., Giordano, G. & Furlong, C. E. (2011) Pharmacological and dietary modulators of paraoxonase 1 (PON1) activity and expression: the hunt goes on. Biochemical Pharmacology. Vol. 81. Issue 3, pp 337–344
Demir, B., Demir. S., Atamer, Y., Guven, S., Atamer, A. & Kocyigit, Y. (2011) Serum levels of lipids, lipoproteins and paraoxonase activity in pre eclampsia. The Journal of International Medical Research. Vol. 39, pp 1427 1431.
Diaz Vivancos, P. Wolff, T. Markovic, J. Pallard´ o, F. V. & C.H. Foyer, (2010): “A nuclear glutathione cycle within the cell cycle,” Biochemical Journal. Vol. 431. Issue 2, pp 69–178.
Draganov, D.I., Teiber, J.F., Speelman, A., Osawa, Y., Sunahara, R. & La Du, B.N. (2005) Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. Journal of Lipid Research. Vol.46. Issue 6, pp 1239-1247.
Erel O. (2005) A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 38(12):1103-11.
Genc, H., Uzun, H., Benian, A., Simsek, G., Gelisgen, R. and Madazli, R. (2011) Evaluation of oxidative stress markers in first trimester for assessment of preeclampsia risk. Arch Gynecol Obstet. Vol. 284, pp 1367 1373.
Ho, Y. S., So, K. F. and Chang, R. C. (2010) Anti-aging herbal medicine--how and why can they be used in aging-associated neurodegenerative diseases? Ageing Res Rev. Vol. 9, pp 354–362
Hubel, C.A., McLaughlin, M.K. & Evans, R.W. (1996): Fasting serum triglycerides, free fatty acids, and malondialdehyde are increased in preeclampsia, are positively correlated, and decrease within 48 hours post partum. Am J Obstet Gynecol. Vol. 174, pp 975 – 982
Kim, Y.J., Park, H. & Lee, H.Y. (2007) Paraoxonase gene polymorphism, serum lipid, and oxidized low-density lipoprotein in preeclampsia. European Journal of Obstetrics Gynecology and Reproductive Biology. Vol. 133. Issue 1, pp 47–52.
Laivuori, H., Gallaher, M., Collura, L., Crombleholme, W., Markovic, N. & Rajakumar, A, (2006) Relationships between maternal plasma leptin, placental leptin mRNA and protein in normal pregnancy, preeclampsia and intrauterine growth restriction without preeclampsia. MHR: Basic Science of Reproductive Medicine. Vol. 12. Issue 9, pp 551–556.
Mackness, M. I., Mackness, B. & Durrigton, P. N. (2002) Paraoxonase and coronary heart disease. Atherosclerosis. Vol. 3, pp 49–55.
Mohanty, S., Sahu, P. K., Mandal, M. K. & Mohapatra, P. C. (2006) Panda A. Evaluation of oxidative stress in pregnancy induced hypertension. Indian J Clin Biochem. Vol. 21, Issue 1, pp 101-105.
Précourt, L.P., Amre, D., & Denis, M.C. (2011). The three-gene paraoxonase family: physiologic roles, actions and regulation. Atherosclerosis. Vol. 214, Issue 1, pp 20–36.
Sarandöl, E., Safak, O., Dirican, M. & Uncu, G. (2004) Oxidizability of apolipoprotein B containing lipoproteins and serum paraoxonase/arylesterase activities in preeclampsia. Clin Biochem; Vol. 37, pp 990 6.
Steegers, E.A., Von Dadelszen, P., Duvekot, J.J., & Pijnenborg R. (2010): Pre-eclampsia. Lancet; Vol. 376, pp 631–644.
Uzun, H., Benian, A., Madazli, R., Topçuoğlu, M.A., Aydin, S., & Albayrak, M. (2005) Circulating oxidized low-density lipoprotein and paraoxonase activity in preeclampsia. Gynecologic and Obstetric Investigation. Vol. 60, Issue 4, pp 195–200.
Varshney, R. and Kale, R.K. (1990) Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. Int J Radiat Biol. 58(5):733-43.
Yaghmaei, M., Hashemi, M., Azarian, A., Moazeni Roodi, A., Mokhtari, M., & Naghavai, A. (2011) Association of L55M and Q192R polymorphisms of paraoxonase 1 gene with preeclampsia. Arch Med Res. Vol. 42, pp 324 328.
Yin, H., Xu, L., and Porter, N.A. (2011) Free radical lipid peroxidation: mechanisms and analysis. Chemical Reviews. 111(10):5944–5972.
Zabul, P., Wozniak, M., Slominski, A.T., Preis K., Gorska M., Korozan M., Wieruszewski J., Zmijewski M.A., Zabul E., & Tuckey R. (2015): A proposed molecular mechanism of high-dose vitamin d3 supplementation in prevention and treatment of Pre-eclampsia. Int. J. Mol. Sci. Vol. 16, pp 13043–13064.
Downloads
Published
How to Cite
Issue
Section
License
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution (CC-BY) 4.0 License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
