Occurrence and Distribution of Aflatoxin in Maize from Selected Counties, Eastern Region, Kenya
DOI:
https://doi.org/10.47941/jap.438Keywords:
Aflatoxin, Distribution, Maize, Occurrence, StoresAbstract
Purpose: The study aimed to assess the occurrence and distribution of aflatoxin contamination on dry maize in different types of stores in Meru, Embu, Isiolo, Makueni and Machakos Counties of Eastern region of Kenya.
Methodology: Automatic spear sampler was used to collect maize samples from each bag at even intervals. 280 maize samples were collected from 29 stores in five Counties. 100 g of each maize sample was ground, resampled into 50g, blended, extracted, centrifuged, filtered and a quantified for Aflatoxin B1, B2, G1 and G2. Samples were prepared and extracted with methanol/water. The bulky of the samples were analyzed with enzyme-linked immunoassay test kits. Confirmation of positive samples was done with high performance liquid chromatography (HPLC) coupled with fluorescence detector. Data analysis was done with SPSS and Microsoft excel.
Findings: Maize samples from Counties in eastern region of Kenya had significantly high levels of (93.10%) aflatoxin contamination. The mean values for aflatoxin B1, B2, G1 and G2 were: 50.08± 4.42, 17.26±1.08, 30.17±2.06 and 10.54± 1.52 (ng/g) in that order. Only nine samples had total aflatoxin within the accepted limit for human consumption of 15 ng/g. The highest total aflatoxin contamination recorded was 198.45ng/g in Makueni county and the lowest recorded was 8.76ng/g in Embu county. Makueni and Embu had mean values for aflatoxin B1, B2, G1 and G2 being (83.07±7.53, 22.15± 1.36, 49.38±3.11, 20.52± 0.70 ng/g) and (18.71 ±2.63, 8.07 ±0.64, 17.02 ±1.38, 8.86 ±1.62 ng/g). Makueni NCPB depot had the highest mean contamination with aflatoxin B1 of 92.67± 5.78 ng/g and Embu had the lowest with 6.26 ± 4.14 ng/g. All the county markets recorded high aflatoxin B1 contamination with exception of Embu county which had a mean of 4.0 ±0.84, Makueni (83.67± 10.42 ng/g), Isiolo (51.27± 32.29 ng/g), Meru (46.02± 23.88 ng/g) and Machakos (36.34± 26.27 ng/g). The stores had aflatoxin load varied from on store to the other and county to county.
Unique contribution to theory, policy and practice: The counties in the region had high occurrence and distribution of aflatoxin B1, B2, G1 and G2 in maize in all stores where samples were picked. Location for maize stores should be in areas with low levels of carbon dioxide because mycotoxins are produced under aerobic conditions. The design for maize threshing machines should not course shocks, breakage and cracks on maize grains to decrease chances of mycotoxins infestation during their storage.Downloads
References
Alexa, E., Dehelean, C. A., Poiana, M. A., Radulov, I., Cimpean, A. M., Bordean, D. M., Pop, G. (2013). The occurrence of mycotoxins in wheat from western Romania and histopathological impact as effect of feed intake. Chemistry Central Journal, 7(1).
Azziz-Baumgartner E, Lindblade K, Gieseker K, Rogers HS, Kieszak S, Njapau H, Schleicher R, McCoy LF, Misore A, DeCock K, (2005). Case-control study of an acute aflatoxicosis outbreak, Kenya, 2004. Environ Health Perspect.;113:1779-1783.
Centers for Disease Control and Prevention. Outbreak of aflatoxin poisoning - eastern and central provinces, Kenya, January-July 2004. MMWR Morb Mortal Wkly Rep. 2004; 53:790-793.
Cotty, P. J., & Jaime-Garcia, R. (2007). Influences of climate on aflatoxin producing fungi and aflatoxin contamination. International Journal of Food Microbiology, 119(1-2), 109-115.
Daniel J.H, Lewis LW, Redwood YA, Kieszak S, Breiman RF, Flanders WD, Bell C, Mwihia J, Ogana G, Likimani S, (2011). Comprehensive assessment of maize aflatoxin levels in Eastern Kenya, 2005-2007. Environ Health Perspect.119:1794-1799.
Eze, U. A., and Okonofua, F. E. (2015). High prevalence of male infertility in Africa: Are mycotoxins to blame? African Journal of Reproductive Health, 19(3), 9-17.
Gachara, G. W., Nyamache, A. K., Harvey, J., Gnonlonfin, G. J. B., & Wainaina, J. (2018). Genetic diversity of Aspergillus flavus and occurrence of aflatoxin contamination in stored maize across three agro-ecological zones in Kenya. Agriculture and Food Security, 7(1).
George Marechera and Josphe Ndwiga (2014). Farmer perceptions of aflatoxin management strategies in lower Eastern Kenya. Journal of Agricultural Extension and Rural Development, 6(12), 382-392.
Gnonlonfin, G. J. B., Hell, K., Adjovi, Y., Fandohan, P., Koudande, D. O., Mensah, G. A., Brimer, L. (2013). A Review on Aflatoxin Contamination and Its Implications in the Developing World: A Sub-Saharan African Perspective. Critical Reviews in Food Science and Nutrition, 53(4), 349-365.
Gong Y.Y., Wilson, S., Mwatha, J.K., Routledge, M.N., Castelino, J.M., Zhao, B., Kimani, G., Kariuki, H.C., Vennervald, B.J., Dunne, D.W. and Wild, C.P., 2012. Aflatoxin exposure may contribute to chronic hepatomegaly in Kenyan school children. Environmental Health Perspectives 120: 893-896.
Hell K, Cardwell K., Poehling H (2003). Relationship between management practices, fungal infection and aflatoxin for stored maize in Benin. J. Phytopath., 151: 690-698.
Houssou, P. A., Ahohuendo, B. C., Fandohan, P., Kpodo, K., Hounhouigan, D. J., & Jakobsen, M. (2009). Natural infection of cowpea (Vigna unguiculata (L.) Walp.) by toxigenic fungi and mycotoxin contamination in Benin, West Africa. Journal of Stored Products Research, 45(1), 40-44.
Kaaya, A. N., Kyamuhangire, W., & Kyamanywa, S. (2006). Factors affecting aflatoxin contamination of harvested maize in the three agroecological zones of Uganda. Journal of Applied Sciences, 6(11), 2401-2407.
Kang'ethe, E. K., Gatwiri, M., Sirma, A. J., Ouko, E. O., Mburugu-Musoti, C. K., Kitala, P. M., ... & Joutsjoki, V. (2017). Exposure of Kenyan population to aflatoxins in foods with special reference to Nandi and Makueni counties. Food Quality and Safety, 1(2), 131-137.
Kitya S., G. D., Lubega, A., Ogwal-Okeng, J., W., W., & B., D. (2013). Review of the Biological and Health Effects of Aflatoxins on Body Organs and Body Systems. In Aflatoxins - Recent Advances and Future Prospects.
Kumar, P., Mahato, D. K., Kamle, M., Mohanta, T. K., & Kang, S. G. (2017, January 17). Aflatoxins: A global concern for food safety, human health and their management. Frontiers in Microbiology. Frontiers Media S.A.
Lewis, L., Onsongo, M., Njapau, H., Schurz-Rogers, H., Luber, G., Kieszak, S., Nyamongo, J., Backer, L., Dahiye, A.M., Misore, A., DeCock, K. and Rubin, C., 2005. Aflatoxin contamination of commercial maize products during an outbreak of acute aflatoxicosis in Eastern and Central Kenya. Environmental Health Perspectives 113: 1763-1767.
Mahuku, G., Nzioki, H. S., Mutegi, C., Kanampiu, F., Narrod, C., & Makumbi, D. (2019). Pre-harvest management is a critical practice for minimizing aflatoxin contamination of maize. Food Control, 96, 219-226. https://doi.org/10.1016/j.foodcont.2018.08.032
Medina, A., Rodriguez, A., & Magan, N. (2014). Effect of climate change on Aspergillus flavus and aflatoxin B1 production. Frontiers in Microbiology. Frontiers Research Foundation.
Milani, J. M. (2013). Ecological conditions affecting mycotoxin production in cereals: A review of Veterinarian Medicine 58(8): 405 - 411.
Mutegi, C.K., Cotty, P.J. and Bandyopadhyay, R., 2018. Prevalence and mitigation of aflatoxins in Kenya (1960-to date). World Mycotoxin Journal, 11(3), pp.341-357.
Mutunga E, Ndungu C. and Muendo P. (2017). Smallholder Farmers Perceptions and Adaptations to Climate Change and Variability in Kitui County, Kenya. Journal of Earth Science & Climatic Change, 08(03). https://doi.org/10.4172/2157-7617.1000389
Negash D. (2018). A review of aflatoxin: occurrence, prevention, and gaps in both food and feed safety. Journal of Applied Microbiological Research 1: 35-43.
Nyangi C., Beed, F., Mugula, J. K., Boni, S., Koyano, E., Mahuku, G., Bekunda, M. (2016). Assessment of pre-harvest aflatoxin and fumonisin contamination of maize in Babati district, Tanzania. African Journal of Food, Agriculture, Nutrition and Development, 16(3), 11039-11053.
Okoth, S.; Nyongesa, B.; Ayugi, V.; Kang'ethe, E.; Korhonen, H.; Joutsjoki, V. (2012). Toxigenic Potential of Aspergillus Species Occurring on Maize Kernels from Two Agro-Ecological Zones in Kenya. Toxins, 4, 991-1007.
Olarte, R. A., Horn, B. W., Dorner, J. W., Monacell, J. T., Singh, R., Stone, E. A., & Carbone, I. (2012). Effect of sexual recombination on population diversity in aflatoxin production by Aspergillus flavus and evidence for cryptic heterokaryosis. Molecular Ecology, 21(6), 1453-1476.
Ongoma, V., 2013. A review of the effects of climate change on occurrence of aflatoxin and its impacts on food security in semiarid areas of Kenya. International Journal of Agricultural Science Research 2: 307-311.
Owuor Lalah, J., Omwoma, S., & A.O. Orony, D. (2020). Aflatoxin B1: Chemistry, Environmental and Diet Sources and Potential Exposure in Human in Kenya. In Aflatoxin B1 Occurrence, Detection and Toxicological Effects.
Paica, A., Ene, C. I., & Stefan, L. A. (2013). Toxigenic fungi of Aspergillus genus and corn crop vulnerability to infection produced by these. Romanian Journal for Plant Protection, 6, 77-81.
Probst, C., Bandyopadhyay, R., & Cotty, P. J. (2014). Diversity of aflatoxin-producing fungi and their impact on food safety in sub-Saharan Africa. International Journal of Food Microbiology, 174, 113-122. https://doi.org/10.1016/j.ijfoodmicro.2013.12.010
Reddy, K. R., Seepaul, R., Henry, W. B., Gajanayake, B., Lokhande, S. and Brand, D. (2014). Maize (Zea mays L) yield and aflatoxin accumulation responses to exogenous glycinebetaine application. International Journal of Plant Production 8(2): 1735 - 6814
Ren, Y., Jin, J., Zheng, M., Yang, Q., & Xing, F. (2020). Ethanol Inhibits Aflatoxin B1 Biosynthesis in Aspergillus flavus by Up-Regulating Oxidative Stress-Related Genes. Frontiers in Microbiology, 10.
Shephard, G. S. (2008). Impact of mycotoxins on human health in developing countries. Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 25(2), 146-151.
Wassmann, R., Jagadish, S. V. K., Sumfleth, K., Pathak, H., Howell, G., Ismail, A., "¦ Heuer, S. (2009). Chapter 3 Regional Vulnerability of Climate Change Impacts on Asian Rice Production and Scope for Adaptation. Advances in Agronomy.
Wu, F., 2010. The global burden of disease caused by foodborne aflatoxin. WHO Commissioned Report. Geneva: World Health Organization Food Borne Disease Burden Epidemiology Reference Group
Xu, Y., Gong, Y. Y., & Routledge, M. N. (2018). Aflatoxin exposure assessed by aflatoxin albumin adduct biomarker in populations from six African countries. World Mycotoxin Journal. Wageningen Academic Publishers.
Yard, E.E., Daniel, J.H., Lewis, L.S., Rybak, M.E., Palikov, E.M., Kim, A.A., Montgomery, J.M., Bunnell, R., Abudo, M.U., Akhwale, W., Breiman, R.F. and Sharif, S.K., 2013. Human aflatoxin exposure in Kenya, 2007: a cross-sectional study. Food Additives and Contaminants Part A 30: 1322-1331.
Zain, M. E. (2011). Impact of mycotoxins on humans and animals. Journal of Saudi Chemical Society, 15(2), 129-144. https://doi.org/10.1016/j.jscs.2010.06.006
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Copyright (c) 2020 Nicholas M. Jacob, Shem O. Wandiga, David K. Kariuki, Vincent O. Madadi
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