LAB-SCALE PRODUCTION OF ANTI-SCHISTOSOMA MONOCLONAL ANTIBODIES BY HYBRIDOMA CULTURING IN SERUM FREE MEDIUM: ENHANCEMENT OF GROWTH, CRYOPRESERVATION SURVIVAL RATE, AND DIAGNOSTIC ACCURACY.
DOI:
https://doi.org/10.47941/ijhs.972Keywords:
lab-scale production, monoclonal antibodies, hybridoma, serumAbstract
This study was performed to evaluate the effect of two commercially available serum independent culture media; serum free medium (SFM) and chemically defined medium (CDM), on the growth rate, antibody productivity and post adaptation cryopreservation and revival reactivity of hybridoma cells compared to the conventional serum based medium (SBM). In addition, the diagnostic efficacy of MAbs secreted in each culture medium was evaluated by testing their performance in sandwich ELISA for antigen detection. Anti- Shistosoma mansoni soluble egg antigen hybridoma cell line (7A/8F) secreting previously characterized IgG Kappa mAbs, recognizing repetitive epitopes on Shistosoma mansoni soluble egg antigen, were retrieved and propagated in each of the three forementioned media, growth rate and viability were assessed post culturing in each media. The data collected from this study indicated that MAbs secreted from hybridoma cells cultured in SFM were the most abundant, easiest to purify, and the most effective in antigen detection by sandwich ELISA, than those produced in the other two media. Moreover, SFM provided a high quality medium for lab-scale production of MAbs. Moreover, combination of fresh and conditioned medium with DMSO 7.5% was the most promising formulation for the cryopreservation of hybridoma cells cultivated in serum independent media (SFM or CDM).
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