Bacteriological and Biochemical Study of Natural Honey Available on the Mbujimayi Market
DOI:
https://doi.org/10.47941/ijhmnp.3170Keywords:
Bacteriological and Biochemical Study, Natural Honey, MbujimayiAbstract
Purpose: to study the sterility and biochemical composition of natural honey available on the market in the town of Mbujimayi.
Methods: This is a descriptive cross-sectional study, carried out from January 1st to April 30th 2023, a period of 4 months. The study was carried out in two laboratories in the Democratic Republic of Congo: the bacteriological laboratory of the Clinique MIBA located in the commune of Kanshi, city of Mbujimayi, province of Kasaï Oriental for the bacteriological study, and the Centre de Recherche Agro-alimentaire (CRAA) located in the commune of Lubumbashi, city of Lubumbashi, province of Haut-Katanga for the biochemical study. The study sample consisted of natural honey from three local origins: Kabinda (Lomami), Sankuru (Lusambo) and Kanyama (Haut Lomami), chosen on the basis of the availability of their honeys on the market in the town of Mbujimayi. The biochemical study consisted in measuring: water content, PH, sugars, proteins, protein identification, and mineral elements by atomic emission spectrophotometry. Germ testing included yeast, staphylococcus aureus, enterobacteria and salmonella.
Results: Bacteriologically, all three samples were sterile. Biochemically, the three honey samples contained four biochemical substances: carbohydrates, proteins, vitamins (B and C) and minerals (copper, iron, manganese, calcium, magnesium, zinc, sodium, potassium, nickel).
Unique contribution to theory, practice and policy: Local honey from Mbujimayi is sterile whatever its origin (all three origins). The biochemical composition was almost identical, with good antioxidant, antibacterial, nutritional and healing properties.
Downloads
References
Baudel M. Apitherapy. Pharmaceutical Sciences. University of Picardy, 2017.
Bendrer N, Messaouda A. Antibacterial effect of some varieties of Algerian honey. University Ibn Khaldoun-Tiaret, 2021.
Şireli S, Saylak M. The effect of storage temperature on some physicochemical properties of flower honey. J Ethn Foods. 2024;11(1):10. doi:10.1186/s42779-024-00229-3
Ghramh H, Ali Khan K, Alshehri A. Antibacterial potential of honeys from the Asian region against selected pathogenic bacteria. Saudi Journal of Biological Sciences, 2018; 26(6):1278–84.
Abdellah F, Makhloufi C, Boukraa L, Hammoudi Si M, Safa A, Delle N et al. Physicochemical properties, antibacterial and antioxidant activities of two honey varieties from the Algerian Steppe. Journal of Apitherapy and Nature, 2020; 3(2):59–74.
Achouri I, Aboussaleh Y, Sbaibi R, Chemissi H, Bengueddour R. Comparison of physicochemical quality of Ziziphus sp. (Sidr) and Acacia sp. (Samar) honeys consumed in the United Arab Emirates. Innovative Space of Scientific Research Journals, 2015; 10(1):184–91.
Almeida AMM, Oliveira MBS, Costa JG, Valentim IB, Goulart MOF. Antioxidant capacity, physicochemical and floral characterization of honeys from Northeast Brazil. Revista Virtual de Química, 2016.
Amessis N, Amiot MJ, Mansour K. Physicochemical properties and biological activities of some honeys. Food Sciences. University M’hamed Bouguera, 2015; 113 pages.
Bakchiche B, Habati M, Benmebarek A, Gherib A. Physicochemical characteristics, phenolic compound concentrations and antioxidant capacity of four local honey varieties (Algeria). Rev. Mar. Sci. Agron., 2017; 6(1):118–23.
Belhaj O, Oumato J, Zrira S. Physicochemical study of some Moroccan honeys. Rev. Mar. Sci. Agron., 2015; 3(3):71–75.
Benabdallah A. Ecophysiological study, development and importance of medicinal plants of the Mentha genus in the El-Kala National Park (Northeast Algeria). University of Constantine 1, 2017.
Benbareka O, Hafsaoui I. Study of the antibacterial activity of honey harvested in Algeria. University Saad Dahleb-Blida, 2019; 82 pages.
Benkhoucha K, Chenawi K, Mazouni R. Physicochemical and pollen analysis of some Algerian honeys. University Djilali Bounaama of Khemis-Miliana, 2020; 112 pages.
Benzohra A, Ben Saada H. Physicochemical and pollen analysis of honeys produced in different regions. University Djilali Bounaama-Khemis Miliana, 2017.
Boudjelloua R. Contribution to the study of honey consumption in the Tizi-Ouzou region. University Mouloud Mammeri, 2018.
Haskoury R, Kriaa W, Lyoussi B, Makni M. Honeys from Morocco: Physicochemical properties, mineral contents, and antioxidant activities. Journal of Food and Drug Analysis, 2018; 2(6):67–73.
Oskouei ET, Najafi M. Traditional and modern uses of natural honey in human diseases: a review. J. Basic Med. Sci., 2018; 16(7):31–42.
Echchoui A, Benyachou M, El Aissaoui I, Al Ahmad A, Hafidi J, El Mazaiz S, et al. The relevance of honey as an alternative in the treatment of chronic wounds. Ann. Afr. Med., 2014; 7(3).
Kone SGN, Touré S, Bana A, Kone S, Dogba E. Honey in skin wound treatment. Mali Méd., 2016; 31(3).
Mukherjee D, Azamthulla M, Santhosh S, Dath G, Ghosh A, Natholia R et al. Development and characterization of chitosan-based hydrogels as wound dressing materials. J. Drug Deliv. Sci. Technol., 2018; 46:498–510.
Haskoury R, et al. [duplicate of reference 16].
Imtara H, Elamine Y, Lyoussi B. Physicochemical characterization and antioxidant activity of Palestinian honey samples. Food Science and Nutrition, 2018; 6(8):2056–65.
Korichi N, Latamene. Physicochemical and palynological analysis and antibacterial effect of some honeys from Bejaïa. University Abderrahmane Mira of Bejaia, 2017.
Krichen S, Guetatlia I. Evaluation of the antibacterial activity of seven honey samples from Guelma and Tipaza regions. University of Guelma, 2019.
Hallouz MFZ, Mamoun NO. Physicochemical study and evaluation of biological activities of jujube honeys. University Ibn Khaldoun-Tiaret, 2020.
Haderbache L, Annou S, Mohammedi A. Antimicrobial potential of Ziziphus and Euphorbia honeys harvested in a semi-arid region of Algeria and their possible use in soft medicine. Journal of Microbiology, 2020.
Yahiaoui, Soualmia S, Hamadouch S, Nadira. Effect of Sidr honey on the reproductive system and some biochemical parameters, and its impact on wound healing in Wistar albino rats. University Oum El Bouaghi, 2018.
Olivier P. Getting Started in Beekeeping. Afnil-France, 2017; 121 pages.
Laredj H, Rezzoug W. Microbiological and physicochemical characterization of honeys from the Tiaret region of Algeria. Asian Journal of Pharmaceutical Research and Health Care, 2017.
Nascimento K, Sattler JAG, Macedo LFL, González CVS, Pereira MIL, Araújo SE. Phenolic compounds, antioxidant capacity and physicochemical properties of Brazilian Apis mellifera honeys. LWT – Food Science and Technology, 2018; 91:85–94.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Crispin Muamba Mukendi, Claris Mpingiyabu Musangu, Trésor Kabuya Kabamba, Roger Kamwema Shamuana, Séraphin Binene Katulondi, Alidor Mbangila Yombo, Anaclet Tshinyangu Kandanda, André Mutombo Kabamba, Joseph Ngandu Tshilunda, Gabriel Wakunga Warach, Séverin Uwonda Akinja

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution (CC-BY) 4.0 License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.